| x | x-coordinate (subpixel accurate) |
| y | y-coordinate (subpixel accurate) |
| z | z-coordinate (plane where centrosome intensity is maximal) |
| t | Frame Number |
| objID | unique ID of detected object |
| trID | ID of the trajectory to which the object belongs |
| cellName |
P0, P1, … encoded as integer as follows: P0(1),P1(2),AB(3),P2(4),EMS(5),ABp(6),ABa(7),cellst8(8),cellst16(9),cellst32(10),cellst64(11),P3(12), C(13),E(14),MS(15),ABpl(16),ABpr(17),ABal(18),ABar(19), P4(20), D(21), Ca(22),Cp(23),intestine(24), MSa(25),MSp(26),ABprp(27),ABpra(28),ABplp(29),ABpla(30),ABarp(31),ABara(32),ABalp(33),ABala(34) |
| side | 1 – posterior, 2 – anterior |
| NEBD | time point (frame number) where the nuclear envelope breakdown (NEBD) occurs in the respective cell |
| normTime | time (in seconds) relative to NEBD |
| radius | centrosome radius [nm] (based on elliptical Gaussian fit, corrected for non-Gaussian profiles, corrected for the point spread function) |
| totalInt2D | total intensity in the centrosome’s z-plane [arbitrary units] |
| bgLevel | average cytoplasmic background intensity in the centrosome’s z-plane [arbitrary units] |
| mx | x-coordinate (subpixel accurate) |
| my | y-coordinate (subpixel accurate) |
| sigma_p | standard deviation along the principal (i.e. major) axis |
| sigma_m | standard deviation along the minor axis |
| gaussMult | peak height of the Gaussian |
| gaussAdd | additive offset (i.e. background level in the immediate vicinity of the centrosome) |
| gaussRho | orientation of the major axis [in degrees] |
| gaussAdd | additive offset (i.e. background level in the immediate vicinity of the centrosome) |
| fromMixtureFit |
0 – simple fit (one Gaussian) 1 – mixture fit tried, but not accepted 2 – the Gaussian parameters are based on a two-component mixture fit |